If you want to configure for just a particular site/sub-directory, it can also go in the Apache Directives for required web in ISPConfig2.
Unsure about ISPConfig3
fwiw I just went through this on a test server, it seems to me I had to manually restart or reload apache to get the new directory directive to be recognized and the forbidden message to go away. I put the directive in the additional apache directives in ispconfig3. This was done on centos 5.3
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