You can not use .htaccess files for the site, because the use of .htaccess files is not allowed on your system.
To allow .htaccess files for a single site, enter the following to the apache directives field on the basis tab of the web within ISPConfig:
Note: web# is the webnumber as created by ISPConfig.
Allow the use of .htaccess files on your server globaly, by changing AllowOverride None into AllowOverride All within your Apache configuration file.
Restart Apache afterwards.
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